I'm now deploying an django app with nginx and gunicorn on ubuntu 12.

And I configure the nginx virtual host file as below:

server {
    listen 80;
    server_name mydomain.com;
    access_log  /var/log/nginx/gunicorn.log;
location / {
    proxy_pass http://127.0.0.1:8000;
    proxy_set_header Host $host;
    proxy_set_header X-Real-IP $remote_addr;
    proxy_set_header X-Forwarded-For $proxy_add_x_forwarded_for;
}

location /static/ {
    root /var/www/django/ecerp/erp/static/;
}
}

I can request the django well, but when request a static file, it response with 404 status.

I'm sure the root path of static file is correct.

Can anyone help?

You should use alias instead of root. root appends the trailing URL parts to your local path (e.g. http://test.ndd/trailing/part, it will add /trailing/part to your local path). Instead of that, alias does exactly what you want: when http://test.ndd/static/ is requested, /static is mapped to your alias exactly, without appending static again.

For example:

location /static {
    alias /var/www/django/ecerp/erp/static/;
}

And if file /var/www/django/ecerp/erp/static/foo.html exists then going to /static/foo.html will return its contents.